Immobilization of biologically active species on PA‐6 foils treated by a dielectric barrier discharge

In the field of biomaterials and biomedical devices, surface activation has been focused on creating functional groups capable of preferential adsorption of biologically active species (proteins, enzymes, cells, drugs, etc.). In this way an interface can be created between the synthetic material and the biological medium, with the aim of increasing the compatibility of the implant with the human organism. In our experiments a dielectric barrier discharge (DBD), in helium at atmospheric pressure, was used as the source of energy capable of creating active centers that render the functionalized surface favorable to immobilization of biological molecules. Retention of immunoglobulin (IgG) and heparin biomolecules on polyamide‐6 (PA‐6) surfaces after treatment by the DBD was analyzed by atomic force microscopy, adhesion evaluation, and measurement of the contact angle titration in order to assess this incorporation on the treated surfaces. The marked adsorption of the biomolecules on the active sites created by DBD on the exposed surfaces also was related to a complex set of processes, such as enhanced roughness, increased surface wettability, and modified distribution of cationic and anionic groups on the treated surfaces. All these factors could promote interfacial interactions between the specific groups of the biomolecules existing in the biological medium and the type of cationic and/or anionic groups present on the surface. The efficiency of the DBD treatment showed that the DBD technique is useful for preactivation of the polymer surface for immobilization of other biologically active species (such as drugs and enzymes). © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 90: 1985–1990, 2003     

基于响应曲面模型的泵站进水池参数优化方法研究

泵站进水池的结构参数,特别是吸水喇叭管参数取值对泵站整体流态影响很大。本文基于响应曲面模型和CFD数值模拟,以垂直布置的吸水喇叭管悬空高、后壁距和淹没深度为设计变量,以进水池压力场、速度场和涡量场评价指标的加权函数为目标函数,进行2因素和3因素的优化设计分析。研究结果表明,变量之间对进水池流态的交互作用较大,淹没深度对最优悬空高、后壁距的影响不可忽略,且淹没深度与悬空高的交互作用(P=0.0019)较其与后壁距的交互作用(P=0.0696)大。针对本文垂直布置型式的吸水喇叭管,推荐最优参数组合是悬空高0.77 D,后壁距0.37 D,淹没深度2.19 D。在该组合下实际值与预测值误差不大于4.82%,与泵站设计规范推荐值相比,其计算域水力损失降低0.98%,流速分布均匀度提高5.92%,机组纵剖面涡量分布特征值降低3.1倍,大大改善了进水池的流态。这表明所建立的二次多项式响应面模型能够较精确地表示设计变量与目标函数之间的关系,基于响应曲面模型的优化设计方法可有效用于泵站进水流场的流态优化。     

甲氧胺盐酸盐的含量测定方法

提出了以酚酞为指示剂，氢氧化钠为滴定剂测定甲氧胺盐酸盐含量的方法。测定了甲氧胺盐酸盐的离解常数Ka，考察了干扰因素对测定的影响。结果表明，干扰物质NH4Cl在样品中的含量小于5％不影响测定。该方法具有简便、快速，准确等特点,测定了数批样品结果较满意。     

Determination of the pKa values of titratable groups of an antigen-antibody complex, HyHEL-5-hen egg lysozyme

The titration behavior of the ionizable residues of the HyHEL-5–henegg lysozyme complex and its individual components has beenstudied using continuum electrostatic calculations. Severalresidues of HyHEL-5 had pK_a values shifted away from model valuesfor isolated residues by more than three pH units. Shifts awayfrom the model values were smaller for the residues of hen egglysozyme. A moderate variation in the pK_a values of the titratablegroups was observed upon increase of the ionic strength from0 to 100 mM, amounting to 1–2 pH units in most cases.Under physiological conditions, the net charge of HyHEL-5 wasopposite that for hen egg lysozyme. Several residues, includingthose involved in the Arg–Glu salt bridges that have beenproposed to be important in antibody-antigen binding, had pK_avalues that were changed significantly upon binding. The maintitration event upon antibody-antigen binding appears to beloss of a proton from residue GluH50 of the Fv molecule. Thelimitations of our calculation methods and the role they mightplay in the design of antibodies for use in assays, sensorsand separations are discussed     

云环境下基于神经网络和群搜索优化的资源分配机制

在云环境下,各种闲置资源可以通过池化形成资源池,进而利用虚拟化技术将资源池中的不同资源组合以服务的形式提供给用户使用,因此需要合理而有效的机制来分配资源.针对云环境下资源的特点,将经济学和智能方法相结合,提出了一种基于双向组合拍卖的智能资源分配机制.在该机制中,提出了基于体验质量（quality ofexperience,简称QoE）的威望系统,引入威望衰减系数和用户信誉度,降低拍卖中恶意行为造成的影响,为资源交易提供QoE 支持.对拍卖中的竞价决策,综合考虑多种因素,提出了基于BP 神经网络的竞标价格决策机制,不仅可以合理确定竞标价,而且使价格可以动态适应市场变化.最后,由于组合拍卖胜标确定问题是NP 完全的,因此引入群搜索优化算法,以市场盈余和总体威望为优化目标,得到资源分配方案.仿真研究结果表明,该机制是可行和有效的.     

含氯离子废水化学需氧量分析方法比较

含氯离子废水化学需氧量的分析方法的研究是当前环境监测研究的热点之一，各种文献报道也较多，但各自使用的效果不一。采用试验的方法对含氯离子废水中CODCr的分析进行了初步研究，比较了硫酸汞加入法、稀释法、硝酸银滴定法、氯气校正法、氯离子校正法等方法在不同化学需氧量不同氯离子浓度下的分析结果，利用分析结果与配置的标准溶液浓度相比较，分析了各种方法的优缺点，结果表明硝酸银滴定法是比较理想的适应范围较广的方法。     

基于神经网络的PP/CaCO3复合材料的力学性能预测

研究利用BP网络建立了对PP/CaCO3复合材料性能进行预测的模型,并在此基础上建立了PP/CaCO3复合材料拉伸强度、冲击强度和复合态结构之间的关系.结果表明,该模型有着较好可信度,与实验现象也有着较好的符合;利用该模型证明了分散相粒子粒间距、EPDM层厚度是影响其材料冲击强度,并决定其脆-韧转变的最重要因素,并证明了EPDM层厚度的临界值大约在0.05μm左右.     

乙二醇与马来酸酐酯化反应的研究

优化了乙二醇与马来酸酐的酯化反应,得出了马来酸酐与乙二醇的最佳摩尔比为2.1：1,反应时间为1h,催化剂质量分数为1%,同时反应以丙酮为溶剂.     

蛋壳中钙镁含量的测定

分别采用酸碱滴定法、配位滴定法和氧化还原滴定法测定了普通鸡蛋壳的钙镁总量，并对上述三种方法进行了比较，从中选择出较适合教学的方法为配合滴定法。采用配位滴定法分别测定了普通鸡蛋壳、山鸡蛋壳和乌鸡蛋壳的钙镁总量分别为95．34％、97．03％、83．28％，从中选择出较适合教学的鸡蛋壳为普通鸡蛋壳。     

氮三甲撑膦酸中氯离子含量测定方法的研究

在酸性条件下,用Ag~ 沉淀氮三甲撑膦酸溶液中的Cl~-,然后以淀粉和KIO_3为指示剂,用标准I~-溶液滴定过量的Ag~ 。测定准确,方法灵敏,可靠和方便。克服了莫尔法和佛尔哈德法不适用于该测定体系的困难。